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Contact Admin. The type species, S. In a polyphasic approach field isolates and reference strains of S. Based on growth-independent physiological profiling using VITEK2-compact, API ZYM and the Micronaut system fastidious growth-related difficulties could be overcome and streptobacilli could definitively be typed despite generally few differences.
While differing in their isolation sites and dates, S. Spectroscopic methods facilitated differentiation of S. Sequencing of 16S rRNA gene as well as functional genes groEL , recA and gyrB revealed only little intraspecific variability, but generally proved suitable for interspecies discrimination between all three taxa and two groups of divergent isolates. For almost 90 years, the genus Streptobacillus [ 1 ] S.
The infection is predominantly transmitted through rat bites, scratches or by direct or indirect contact with rat urine [ 4 — 6 ]. Despite its wide distribution in natural hosts S. In the last few years Streptobacillus -like organisms have been observed beside S.
The present study aimed to compare 29 field isolates and reference strains of S. In contrast to earlier investigations [ 13 — 15 ] a spectrum of methods was employed to overcome known diagnostic difficulties due to the fastidious growth of this microorganism. The present study included 29 members of the genus Streptobacillus from different host species which cover isolations of S. From seven Japanese rat strains there was only DNA available.
For the growth of Streptobacillus spp. Physiological characteristics were obtained by an individual panel of eleven discriminatory reactions designed for the identification of Streptobacillus spp. Presumptive physiological characterization further employed standard microbiological procedures: Haemolytic properties of the bacteria were observed on SBA.